Fenestra micro-CT Imaging Agents: Hepatocyte Selective, Long Residence Time in Blood and Completely Cleared

Fenestra® is a licensed technology that solves several key problems associated with micro-CT imaging. This biochemically activated emulsion is comprised of iodinated lipids that provide contrast enhancement and a novel oil-in-water lipid emulsion that selectively localizes the lipids for small animal imaging. The unique Fenestra® formulation is biodegradable and completely eliminated by hepatocyte metabolism. 

Here is a list (with clickable web links) of our Fenestra imaging Agents for In-Vivo imaging:

Fenestra® VC is a refined version of Fenestra® LC in which the surface of the lipid emulsion particles is modified so as to alter the recognition of the particle by the receptors on hepatocytes that are responsible for its uptake into the liver. With Fenestra VC, the delayed uptake by liver cells produces an agent with superior blood pool imaging properties that last for several hours after injection. Moreover, the agent remains truly intravascular so long as the endothelial integrity of the vessel is maintained. Like its liver-selective counterpart, Fenestra VC is eventually metabolized and eliminated through the hepatobiliary system.

Because of their comparatively long and stable in vivo residence times (up to several hours), the Fenestra® products have shown considerable promise for use in microCT imaging procedures. The numerous benefits provided by the Fenestra technology will play an instrumental role in facilitating the implementation of microCT imaging as an increasingly important and popular component of both basic research and commercial drug development.

Some examples of applications …

Fenestra LC Diabetes

Detection, staging and monitoring of mouse model of Diabetes with Fenestra LC

Video courtesy of Dr. Brian Ross, University of Michigan. Movie of bone and enhanced-blood isosurfaces in a mouse thoracic CT volume acquired with a Bruker Skyscan 1176 (~35um voxel size) with a 200 uL dose of MediLumine’s Fenestra VC injected in C57BL/6 female mouse.

Fenestra LC User Guide with MicroCAT II Imaging Hardware

Coronal view (left image) and 3D reconstruction (right image) of male Balb/c mouse with hepatic CT-26 adenocarcinoma 2 hr after IV injection of Fenestra LC. The CT-26 tumor is observed at the lower edge of the liver with a narrow rim of enhanced liver below the tumor and was colorized (purple) in the 3D reconstruction.




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Angiogenesis and Vascular Anatomy

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  5. Gary CK, Park H, Lombardo LW, Piestrup MA, Cremer JT, Pantell RH, Dudchik YI. High Resolution X-ray Microscope. Applied Physics Letters, 90:181111-1-181111-3. 2007.
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  7. Badea CT, Hedlund LW, De Lin M, Boslego Mackel JF, Johnson GA. Tumor imaging in small animals with a combined micro-CT/micro-DSA system using iodinated conventional and blood pool contrast agents. Media Mol Imaging. 2006 Jul-Aug;1(4):153-64.
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Cardiovascular Disease Research

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  2. Sheikh AY, van der Bogt KE, Doyle TC, Sheikh MK, Ransohoff KJ, Ali ZA, Palmer OP, Robbins RC, Fischbein MP, Wu JC. Micro-CT for characterization of murine CV disease models. JACC Cardiovasc Imaging. 2010 Jul;3(7):783-5.
  3. Dinkel J, Bartling SH, Kuntz J, Grasruck M, Kopp-Schneider A, Iwasaki M, Dimmeler S, Gupta R, Semmler W, Kauczor HU, Kiessling F. Intrinsic gating for small-animal computed tomography: a robust ECG-less paradigm for deriving cardiac phase information and functional imaging. Circ Cardiovasc Imaging. 2008 Nov;1(3):235-43.
  4. Detombe SA, Ford NL, Xiang F, Lu X, Feng Q, Drangova M. Longitudinal follow-up of cardiac structure and functional changes in an infarct mouse model using retrospectively gated micro-computed tomography. Invest Radiol. 2008 Jul;43(7):520-9.
  5. Drangova M, Ford NL, Detombe SA, Wheatley AR, Holdsworth DW. Fast retrospectively gated quantitative four-dimensional (4D) cardiac micro computed tomography imaging of free-breathing mice. Invest Radiol. 2007 Feb;42(2):85-94.
  6. Badea CT, Hedlund LW, Mackel JF, Mao L, Rockman HA, Johnson GA. Cardiac micro-computed tomography for morphological and functional phenotyping of muscle LIM protein null mice. Mol Imaging. 2007 Jul-Aug;6(4):261-8.
  7. Nahrendorf M, Badea C, Hedlund LW, Figueiredo J-L, Sosnovik DE, Johnson GA, Weissleder R. High Resolution Imaging of Murine Myocardial Infarction With Delayed Enhancement Cine Micro-CT. Am J Physiol Heart Circ Physiol, 292(6): H3172-8, 2007.
  8. Ford NL, Graham KC, Groom AC, MacDonald IC, Chamber AF, Holdsworth DW. Time-Course Characterization of the Computed Tomography Contrast Enhancement of an Iodinated Blood-Pool Contrast Agent in Mice Using a Volumetric Flat-Panel Equipped Computed Tomography Scanner. Investigative Radiology. 41(4):384-390, 2006.
  9. Badea CT, Bucholz E, Hedlund LW, Rockman HA, Johnson GA. Imaging Methods for Morphological and Functional Phenotyping of the Rodent Heart. Toxicologic Pathology. 34:111-117, 2006.
  10. Badea CT, Fubara B, Hedlund LW, Johnson GA. 4D Micro-CT of the Mouse Heart. Molecular Imaging. 4(2): 110-116, 2005.

Multimodal Imaging (Cancer, Diseases)

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  3. Gabie de Jong, Thijs Hendriks, Rob Bleichrodt, Martin Gotthardt, Eric Visser, Wim Oyen and Otto Boerman. FDG-PET, CT and MRI for the detection of experimental colorectal liver metastases: An exploratory study. J Nucl Med. 2011; 52 (Supplement 1):312
  4. Tsui B, Mok G, Wang Y, Taso A, Bedja D, Yu J, Gabrielson K, Nimmagadda S, Bengal F, Pomper M. High-resolution small animal SPECT/CT imaging of atherosclerotic plaques in ApoE-/- mice using Tc-99m Annexin-V and contrast enhanced CT. J Nucl Med. 2007; 48 (Supplement 2):103P
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  6. Wall JS, Kennel SJ, Paulus M, Gregor J, Richey T, Avenell J, Yap J, Townsend D, Weiss DT, Solomon A. Radioimaging of light chain amyloid with a fibril-reactive monoclonal antibody. J Nucl Med. 2006 Dec;47(12):2016-24.
  7. Avenell, J. Evaluating non-invasive microPET/CT imaging of K-562 myelosarcoma for monitoring tumor growth and angiogenesis in untreated and treated mice. J Nucl Med. 2006; 47 (Supplement 1):556P
  8. Isobe s, Tsimikas S, Zhou J, Fujimoto S, Sarai M, Branks MJ, Fujimoto A, Hofstra L, Reutelingsperger CP, Murohara T, Virmani R, Kolodgie FD, Narula N, Petrov A, Narula J. Noninvasive Imaging of Atherosclerotic Lesions in Apolipoprotein E-Deficient and Low-Density-Lipoprotein Receptor –Deficient Mice with Annexin A5. J Nucl Medicine. 2006 47:1497-1505.
  9. Wall JS, Kennel SJ, Paulus MJ, Gleason S, Gregor J, Baba J, Schell M, Tichey T, O’nuallain B, Donnell R, Hawkins PN, Weiss DR, Solomon A. Quantiitative high-resolution microradiographic imaging of amyloid deposits in a novel murine model of AA amyloidosis. Amyloid: The Journal of Protein Folding Disorders. 12(3):149-156, 2005.
  10. Aykac D, Price JR, Wall JS. 3D Segmentation of the Mouse Spleen in microCT via Active Contours. 2005 IEEE Nuclear Sciences Symposium Conference Record. M03-91, p1542-1545.
  11. Weichert JP, Perlman SB, Lee FT, Johnson CD, Coyle TM, Bennet L. Comparison of a hepatocyte-selective polyiodinated triglyceride CT contrast agent and FDG-PET in the woodchuck model of spontaneous hepatocellular carcinoma. Radiologic Society of North America, 87th Annual Meeting, Chicago IL, 2001.