Angiogenic modulators have been closely studied as a potential therapy for peripheral artery disease and hind limb ischemia. VEGF and other angiomodulating agents have been extensively studied in murine hindlimb models to look at angiogenic effects on healthy and diseased tissues.
More recently, a novel ex vivo technique called microangioCT has been developed with new polymerizing contrast agent called µAngiofil. Contrast enhanced ex vivo microCT imaging with microangiofil allows for quantitative analysis of entire vascular networks as well as correlative histological analysis due to the autofluorescent properties of the contrast agent.
Various visualization modalities of the kidney vasculature and glomeruli. A: the reconstructed 3D stack of the µaCT data set with the focus onto the vasculature of the kidney (bigger vessels are presented in yellow). B: the virtual section through the data set using another transfer function: visualization is focused on kidney tissue (bigger vessels are displayed blue; microvessels and glomeruli are seen as yellow structures; tissue is red). C and C′: the visualization focused onto the glomeruli. C: a volume rendering of a virtual 500 µm-thick slice, as indicated with the white box in the inset in the lower left corner of the image. The white frame (labeled C′) indicates the site with the glomeruli, shown at higher magnification in C′. D and D′: the advanced visualization option: µaCT at a higher resolution (isotropic voxel size = 0.59 µm). D: the inset indicates the virtual section level displayed in D′. The 3D volume rendering of the microvasculature of glomerulus marked in D is displayed in D′.
Cutting-edge microangio-CT: new dimensions in vascular imaging and kidney morphometry. Ruslan Hlushchuk, Cédric Zubler, Sébastien Barré, Carlos Correa Shokiche, Laura Schaad, Raphael Röthlisberger, Monika Wnuk, Christoph Daniel, Oleksiy Khoma, Stefan A. Tschanz, Mauricio Reyes, and Valentin Djonov. American Journal of Physiology-Renal Physiology 2018 314:3, F493-F499. DOI: (10.1152/ajprenal.00099.2017)