PRISM™ In Vivo Imaging System
MediLumine’s EMCCD based PRISM™ uses photon counting for highly sensitive in vivo optical imaging. The imager is able to detect a single photon in a pixel opening a new biological window for bioluminescent imaging. The example below shows PRISM™ in vivo detection of near infrared photons from bioluminescent tumor expressing luciferase.
The system also offers a highly sensitive trans-fluorescent imaging for unparalleled detection of NIR fluorescence due to an EMCCD camera 5x more sensitive than any other camera on market (see comparison below).
Visible light from bioluminescent tumor expressing luciferase (641 ± 37 nm bandpass filter)
Some regions of tumor have average of over 1000 photons per pixel for the full 10 second acquisition.
Near infrared light from bioluminescent tumor expressing luciferase (832 ± 18 nm bandpass filter)
An average of 5 near infrared photons per pixel were detected for a 10 second acquisition (same acquisition parameters and mouse as left image).
In trans-fluorescent mode, PRISM takes a 3D profile of mouse to determine the thickness of tissue penetrated by the laser. A raster scan with NIR laser is subsequently performed to acquire absorption and trans-fluorescent data. 3D profile, absorption and trans-fluorescent data are used by reconstruction software for 3D visualization.
Sensitivity Comparison of PRISM's EMCCD to Competitor's CCD Camera
- Yildian Díaz-Rodríguez, Paulo Cordeiro, Assila Belounis, Sabine Herblot, and Michel Duval. In vitro differentiated plasmacytoid dendritic cells as a tool to induce anti-leukemia activity of natural killer cells. Cancer Immunol Immunother. 2017; 66(10): 1307–1320.
- Oanh Le, Lina Palacio, Gilbert Bernier, Ines Batinic-Haberle, Gilles Hickson, and Christian Beauséjour. INK4a/ARF Expression Impairs Neurogenesis in the Brain of Irradiated Mice. Stem Cell Reports. 2018 Jun 5;10(6):1721-1733.