MediLumine’s EMCCD based PRISM™ uses photon counting for highly sensitive in vivo optical imaging. The imager is able to detect a single photon in a pixel opening a new biological window for bioluminescent imaging.

The example below shows PRISM™ in vivo detection of near infrared photons from bioluminescent tumor expressing luciferase.

The system also offers a real time fluorescence imaging in epi-illumination mode for probe development studies.


  1. Yildian Díaz-Rodríguez, Paulo Cordeiro, Assila Belounis, Sabine Herblot, and Michel Duval. In vitro differentiated plasmacytoid dendritic cells as a tool to induce anti-leukemia activity of natural killer cells. Cancer Immunol Immunother. 2017; 66(10): 1307–1320.
  2. Oanh Le, Lina Palacio, Gilbert Bernier, Ines Batinic-Haberle, Gilles Hickson, and Christian Beauséjour. INK4a/ARF Expression Impairs Neurogenesis in the Brain of Irradiated Mice. Stem Cell Reports. 2018 Jun 5;10(6):1721-1733.
  3. Gaël Moquin-Beaudry, Chloé Colas, Yuanyi Li, Renée Bazin, Jean V. Guimond, Elie Haddad and Christian Beauséjour. Download paper on bioRxiv here: The Tumor-Immune Response Is Not Compromised by Mesenchymal Stromal Cells in Humanized Mice J Immunol November 15, 2019, 203 (10) 2735-2745; DOI:
Near infrared light from bioluminescent tumor expressing luciferase (832 ± 18 nm bandpass filter). An average of 5 near infrared photons per pixel were detected for a 10 second acquisition (same acquisition parameters and mouse as left image).