PRISM IN VIVO IMAGING SYSTEM

MediLumine’s EMCCD based PRISM™ uses photon counting for highly sensitive in vivo optical imaging. The imager is able to detect a single photon in a pixel opening a new biological window for bioluminescence and fluorescence imaging.

In addition, the imaging system is sensitive at high acquisition speeds allowing for real-time imaging in bioluminescent and fluorescence modes.

The example below shows PRISM™ in vivo detection of near-infrared photons from bioluminescent tumor expressing luciferase. In this example, PRISM was able to detect an average of 5 near-infrared photons per pixel for a 10-second acquisition.

User’s of PRISM are finding that the system has an unparalleled combination of real-time imaging and detection of ultra-weak photonic emissions for deeper and earlier in vivo imaging.

Publications

  1. Yildian Díaz-Rodríguez, Paulo Cordeiro, Assila Belounis, Sabine Herblot, and Michel Duval. In vitro differentiated plasmacytoid dendritic cells as a tool to induce anti-leukemia activity of natural killer cells. Cancer Immunol Immunother. 2017; 66(10): 1307–1320.
  2. Oanh Le, Lina Palacio, Gilbert Bernier, Ines Batinic-Haberle, Gilles Hickson, and Christian Beauséjour. INK4a/ARF Expression Impairs Neurogenesis in the Brain of Irradiated Mice. Stem Cell Reports. 2018 Jun 5;10(6):1721-1733.
  3. Gaël Moquin-Beaudry, Chloé Colas, Yuanyi Li, Renée Bazin, Jean V. Guimond, Elie Haddad and Christian Beauséjour. Download paper on bioRxiv here: The Tumor-Immune Response Is Not Compromised by Mesenchymal Stromal Cells in Humanized Mice J Immunol November 15, 2019, 203 (10) 2735-2745; DOI: https://doi.org/10.4049/jimmunol.1900807

 

Tumor cells were injected i.v. and time-lapse in vivo images of lung and liver tumor growth were detected by luminescence (luciferase) in mice without immune reconstitution (no-AT), injected i.v. with 1 × 106 MSCs following Auto-AT of 1 × 107 WBC (Auto-AT) or with AT of WBC and the injection of MSCs (Auto-AT plus MSC). Also shown are images of tumors by fluorescence (mPlum) at sacrifice. ref. https://www.jimmunol.org/content/203/10/2735
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