To gate or not to gate? Attached are high resolution micro-CT images (not gated for motion artefacts) following 0.2 ml injection of Fenestra LDHD in 35 gram mouse scanned with Siemens Inveon (scan parameters: 55 Kv, 500 uA, 36.26 effective pixel size, 2×2 binning) : liver vasculature is clearly visualized after injection (top image) contrast agent is still circulating in liver and mesenteric blood vessels (with some contrast in heart as well) 96 hours after injection (bottom image). Images courtesy of Brenda Baggett and Diego Celdran-Bonafonte, University of Arizona.
Achieving successful tail vein injections is no easy task. As seen in image below, the veins are small and there is a lot of room